S5a protein

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August undefined, 2024

WebNational Center for Biotechnology Information WebJun 15, 2015 · The HIS-tagged human S5a protein was expressed in bacteria following cloning of the entire open reading frame (377 aa) in the pET-21a (Novagen, Billerica, MA) vector. The plasmids were transformed into E. coli BL21 (DE3) and protein expression was induced with 1.0 mM IPTG for 4 hrs at 37°C.

UniProt

WebIdentification of the Ub-like sequence as the S5a-interacting domain of hHR23B raised the possibility that the S5a protein binds to any proteins which are conjugated with ubiquitin. To examine whether this is the case, we performed competition experiments using either free ubiquitin itself or the NH 2-terminal Ub-like domain of hHR23B. WebJul 8, 2024 · Mass spectrometric analyses revealed a highly phosphorylated serine-rich (SR) motif at the C-terminal intrinsically disordered region of the P protein. The Ala-substitution mutant (P S5A) in the SR motif stimulated virus replication, whereas the phosphorylation-mimic mutant (P S5D) facilitated virus transcription. affezionando

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WebOct 14, 2013 · S5a is required for the degradation of p53 but not its E3 ligase Mdm2. Additional work is required to completely define the pathways of proteasomal recognition … WebDec 15, 2009 · Decreased 19S-S5a protein levels and MGO-modified 20S-β2 proteasome subunit in GLO1-knockdown (GL01-KD) and STZ diabetic mice. Kidney lysate protein (40 … WebS5a is one of the integral Ub receptors of the 26S proteasome. It’s N-terminal vWA domain interacts with Rpn9 and Rpn10 on the 26S proteasome. The C-terminal domain of S5a … kum 3レター

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S5a/PSMD4 Antibody Cell Signaling Technology

WebOct 13, 2003 · In this work, we illustrate how the proteasomal subunit S5a regulates hHR23a protein structure. By using NMR spectroscopy, we have elucidated the structure and … WebThe sequence depicted encodes the entire S5a protein beginning with potential initiation Met codons at positions 27 and 54, respectively. The original pACT-derived cDNA, clone 14A, that was isolated based on the interaction of its encoded protein with Id1 begins at codon 34. The entire reading frame encodes a protein of 377 amino acids. kuline ログインWebS5a/Rpn10, a UIM-protein, as a universal substrate for ubiquitination The assay of the activity of ubiquitin (Ub) ligases (E3s) and screens for pharmacological agents that alter … affezionarmi

"WebC5a protein is composed of alpha and beta polypeptide chains, which are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin, which has potent … " - S5a protein

S5a protein

DNA-repair protein hHR23a alters its protein structure upon …

WebFeb 15, 2024 · MIST (protein-protein) - An integrated Molecular Interaction Database RpS5a Reactome - An open-source, open access, manually curated and peer-reviewed pathway … WebParkin is a multidomain E3 ligase associated with autosomal recessive Parkinson disease. The N-terminal ubiquitin-like domain (Ubld) of parkin functions with the S5a proteasomal subunit, positioning substrate proteins for degradation. In addition the parkin Ubld recruits the endocytotic protein Eps15, allowing the E3 ligase to ubiquinate Eps15 distal from its …

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WebJun 15, 2015 · The HIS-tagged human S5a protein was expressed in bacteria following cloning of the entire open reading frame (377 aa) in the pET-21a (Novagen, Billerica, MA) … WebApr 11, 2003 · Total Structure Weight: 9.52 kDa Atom Count: 668 Modelled Residue Count: 85 Deposited Residue Count: 85 Unique protein chains: 1 Display Files Download Files 1P1A NMR structure of ubiquitin-like domain of hHR23B PDB DOI: 10.2210/pdb1P1A/pdb Classification: DNA BINDING PROTEIN Organism (s): Homo sapiens Expression System: …

WebS5A antibody; S5a/antisecretory factor protein antibody; see all. Images. Western blot - Anti-Proteasome 19S S5A/ASF antibody (ab18512) Crude Drosophila extract. Secondary antibody: rabbit polyclonal 1:5000. Please note: Drosophila S5a has an actual mass of 42.6 kDa, however, on the gel it runs at an apparent 54 kDa band. WebApr 10, 2024 · State Key Laboratory of Proteomics, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, 100850 P. R. China. Search for more papers by this author. ... (Figure 5J and Figure S5A,B, Supporting Information). Figure 5. Open in figure viewer PowerPoint.

WebKey features and details Mouse monoclonal [AH1.1] to Proteasome 19S S5A/ASF Suitable for: Flow Cyt, IP Reacts with: Human Isotype: IgG1 You may also be interested in Primary … WebThus, the S5a protein provides a general model for studying recognition of polyubiquitin chains by a 26 S protease component. S5a retains the ability to bind ubiquitin chains even after it has been separated on a SDS-acrylamide gel and transferred to nitrocellulose.

WebSep 24, 1999 · S5a is known as a multiubiquitin chain-binding protein. Since both hHR23 proteins have highly conserved Ub-related sequences on NH 2 termini ( 1, 2 ), we speculated that these sequences are involved in the interaction with S5a. Table I. Protein-protein interactions assessed by the yeast two-hybrid system

WebApr 5, 2024 · As expected, the protein expression of p-ATM was significantly down-regulated by the treatment with KU55933, as was the level of its downstream molecule p-Chk2 (Figure 5e). More importantly, the mRNA levels of SASP-related factors including IL-1α, IL-1β, IL-6, IL-8, CXCL10, and MMP3 were markedly suppressed by KU55933 treatment ( Figure 5f ... affezionarsi in ingleseWebMar 21, 2024 · GeneCards Summary for S100A5 Gene. S100A5 (S100 Calcium Binding Protein A5) is a Protein Coding gene. Diseases associated with S100A5 include … kumaginn 銀行インターネットバンキング法人Web26S proteasome non-ATPase regulatory subunit 4, Antisecretory factor 1, Multiubiquitin chain-binding protein : Clone: S5a-18 : Host: Mouse : Isotype: IgG1 : Immunogen: Recombinant full-length human Rpn10 protein. UniProt ID: P55036 : Gene/Protein Identifier: PSMD4 (gene name) Source: Purified from hybridoma tissue culture supernatant. Species ... kuma3 銀座わぐりへしれけーきWebFeb 19, 2005 · Proteasome subunit S5a contains two ubiquitin-interacting motifs (UIMs) through which it recruits ubiquitylated substrates to the proteasome for their degradation. Here, we report the structure of S5a (196-306) alone and complexed with two monoubiquitin molecules. This construct contains the two UIMs of S5a and we reveal their different ... kulusupa インターホンWebS5a/PSMD4 Antibody detects endogenous levels of total S5a/PSMD4 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly344 of human S5a/PSMD4 protein. affezionarsi coniugazioneWebOct 13, 2003 · The Binding Surfaces of S5a and the UBA Domains Overlap. We used an S5a construct spanning residues M196 to D307 to study the effect of S5a-binding on hHR23a protein structure. S5a (196–307) contains the two ubiquitin-binding domains and was previously shown to bind hHR23a with equal affinity to full-length S5a . affezionarsi sinonimo kumalica ダウンロード